
王娜,女,1980年3月生人,于2008年3月獲中國科學院水生生物研究所遺傳學博士學位,現任中國水產科學研究院黃海水產研究所研究員,碩士生導師。主要從事魚類細胞種質資源保存應用及功能基因組學研究,主持國家自然科學基金面上項目2項、青年基金項目1項,國家863子課題1項,十三五藍色糧倉子課題1項,十四五重點研發計劃課題1項等,發表論文50多篇,其中第一或通訊作者SCI文章28篇、核心期刊2篇,第一發明人獲授權發明專利4項,參編專著2部。作為主要完成人獲得省部級獎勵12項。獲山東省泰山學者青年專家稱號。
聯系電話:15854263035
通訊地址:山東省青島市南京路106號中國水產科學研究院黃海水產研究所
郵政編碼:266071
E-mail:wangna@m.energicube.com
主要研究內容、研究方向
1. 半滑舌鰨等魚類細胞種質資源保存應用技術研究:開發研究適宜于半滑舌鰨等魚類細胞系的高效基因導入技術體系,為魚類功能基因組學研究提供高效平臺;優化半滑舌鰨等魚類的魚類生殖/胚胎干細胞建立及干性特征分析,為魚類優異品系培育及瀕危魚類保護提供重要細胞資源。
2. 半滑舌鰨等魚類性別和生長關聯機制研究:運用組學測序等方法開展半滑舌鰨等性別生長異形魚類在不同性別個體生長-生殖軸的多組學機制分析;利用分子生物學實驗手段開展關鍵基因的表觀、轉錄及蛋白互作機制研究;采用RNA 干擾、基因組編輯等方法揭示關鍵基因在細胞系、組織或成體水平的作用機制。
3. 半滑舌鰨等魚類性別生長異形關鍵基因在基因組編輯育種中的應用潛能研究:借助生物兼容性納米材料、電穿孔技術等,優化建立半滑舌鰨等魚類高效CRISPR-Cas9 基因組編輯技術;開展性別生長異形關鍵基因的基因組編輯敲除或敲入的探索研究,加快高產優質魚類品系的精準培育進程。
代表性論著(10篇以內)
1. Shi R#, Li XH#, Xu XW, Chen ZF, Zhu Y*, Wang N*. Genome-wide analysis of BMP/GDF family and DAP-seq of YY1 suggest their roles in Cynoglossus semilaevis sexual size dimorphism. International Journal of Biological Macromolecules, 2023, 253 (5): 127201.
2. Gong Z#, Zhang Q#, Liu J, Hu G, Chen S*, Wang N*. Establishment, characterization and application in germplasm conservation and disease resistance: An embryonic cell line from Yangtze sturgeon (Acipenser dabryanus). Aquaculture, 2023, 575:739807.
3. Li X, Yang Q, Shi R, Xu X, Chen Z, Chen S, Wang N*. Genome-wide identification of insulin-like growth factor-binding protein family in Chinese tongue sole (Cynoglossus semilaevis) and their responses to sex steroids. Aquaculture 2022; 557: 738346
4. Wang N*, Gao J, Liu Y, Shi R, Chen S*. Identification of crucial factors involved in Cynoglossus semilaevis sexual size dimorphism by GWAS and demonstration of zbed1 regulatory network by DAP-seq. Genomics 2022; 114(3): 110376.
5. Zhu Y, Shi R, Yang Q, Zhang M, Chen S, Wang N*. Two nipped-B like protein A (nipbla) gametologs in Chinese tongue sole (Cynoglossus semilaevis): the identification of alternative splicing, expression pattern and promoter activity analysis, Frontiers in Marine Science 2022; 9: 833070.
6. Wang J, Yang Q, Hu Y, Xu W, Yang Y, Chen S*, Wang N*. Identification of lncRNA-miRNA-mRNA network involved in sexual size dimorphism of Chinese tongue sole (Cynoglossus semilaevis). Frontiers in Marine Science 2022; 9: 795525.
7. Yang Q, Li X, Shi R, Cheng P, Wang N*, Chen S*. The female-biased expression, transcriptional regulation and knock-down effect of insulin-like growth factor binding protein 7 in Chinese tongue sole, Cynoglossus semilaevis. Aquaculture 2022; 551: 737956.
8. Wang N*, Yang Q, Wang J, Shi R, Li M, Gao J, Xu W, Yang Y, Chen Y, Chen S*. Integration of transcriptome and methylome highlights the roles of cell cycle and hippo signaling pathway in flatfish sexual size dimorphism. Frontiers in Cell and Developmental Biology 2021; 9:743722.
9. Wang N*, Wang RK, Wang RQ, Chen SL*. Transcriptomics analysis revealing candidate networks and genes for the body size sexual dimorphism of Chinese tongue sole (Cynoglossus semilaevis). Funct Integr Genomics. 2018;18(3): 327-339.
10. Wang R, Zhang N, Wang R, Wang S, Wang N*. Two skin cell lines from wild-type and albino Japanese flounder (Paralichthys olivaceus): establishment, characterization, virus susceptibility, efficient transfection, and application to albinism study. Fish Physiol Biochem. 2017; 43(6):1477-1486.