Na Wang, Ph.D., Associate Professor, Master's Supervisor.

Address:Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS)

Nanjing Road 106, Qingdao, China

Office:+86-0532-85831605

Email:wangna@m.energicube.com

Personal Profiles:

The bachelor's degree of Na Wang was obtained in 2002 from biology major of Qufu Normal University. From 2002 to 2007, Na Wang entered the Institute of Hydrobiology, Chinese academy of sciences to study for a doctor's degree in Zuoyan Zhu’s laboratory. In 2007, Dr Wang began to work in Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences. Dr Wang’s research focused on marine fish cell culture and important traits gene function study. Dr Wang conducted one National Science Foundation for Young Scholars of China, one 863 sub-project and so on. Also, Dr Wang participated in several projects including 973, 863,the State Key Program of National Natural Science of China,Special Fund for Agro-scientific Research in the Public Interest. From 2007 to now, Dr Wang was one of major members of Taishan Scholar team in Aquatic Germplasm Resources and Biotechnology.

Research Interests:

1) The flatfish major straits genes identification, functional analysis and application potential: Based on the genome information of half smooth tongue sole and Japanese flounder, the key genes and their network regulation mechanism related with the important traits such as growth, immune and sex are our research focus. Another research interest is to establish suitable methods for flatfish fertilized eggs microinjection and improve flatfish gene editing system based on TALEN/Cas9 system. All these study will be helpful to develop the application potential of the key genes in the breeding.

2) The flatfish somatic cells, embryo cells and germline stem cells cultivation and application: The important work is to build multiple cell lines and analyze their application value in the fish pathogenesis and gene function study. Also, to develop the efficient cell transfection method is important for the application feasibility of the somatic cells, embryo cells and germline stem cells in cell engineering breeding way.

Projects and major achievements:

Dr Wang conducted oneNational Science Foundation for Young Scholars of China, one National Science Foundation of China, one 863 sub-project, and oneSpecial Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences.Also, Dr Wang participated in several projects including 973, 863,the State Key Program of National Natural Science of China,Special Fund for Agro-scientific Research in the Public Interest.

1) Dr Wang established and identified several flatfish cell lines, such as turbot kidney cell line, Southern flounder cell line, half smooth tongue sole testis cell line, and half smooth tongue sole head kidney cell line. Their application values in the fish pathogenesis and gene function were also analyzed. Especially, turbot kidney cell line has been used for the study of RNA interference, sub-cellular location, protein activity, gene interaction and so on.

2) The turbot immune related genes GRIM-19, STAT2, STAT3, HSC70 and Hepcidin were cloned and analyzed, and their possible roles against pathogen affection were revealed. The identification and functional analysis of these genes provide comprehensive basis for molecular disease-resistant breeding in aquaculture.

3) The female specific genes CSW3 and CSW4 of Half smooth tongue sole were studied by expression pattern analysis, recombinant proteins identification and TALEN analysis.

4) The transcriptome and miRNA expression pattern between albinism and normal Japanese flounder are analyzed by high-throughput sequencing. There are 235 differentially expressed genes and 46 differentially expressed miRNAs identified. Further GO and KEGG pathway enrichment were also conducted. These studies provide abundant molecular basis for exploring the mechanism of flatfish albinism.

Dr Wang has published over 20 papers in Journals such asDevComp Immunol,Fish Shellfish Immunol, J Fish Dis and so on, of which 6 SCI papers were published as the first author or corresponding author. As well, as the first executers, Dr Wang also obtained two authorized patents.

Selected Publications (* indicated corresponding author):

1. Zhang WT, Liu K, Xiang JS, Zhang LY, Liu WJ, Dong ZD, Li YZ, Li HL, Chen SL,Wang N*. Molecular cloning, expression of, and regulation by thyroid-hormone receptor α A in the half-smooth tongue soleCynoglossus semilaevisduring metamorphosis. J Fish Biol. 2016; 88(5):1693-707.

2. Wang N, Wang X, Yang C, Zhao X, Zhang Y,Wang T, Chen S. Molecular cloning and multifunctional characterization of GRIM-19 (gene associated with retinoid-interferon-induced mortality 19) homologue from turbot (Scophthalmus maximus). Dev Comp Immunol. 2014; 43(1): 96-105.

3. Wang N, Wang XL, Yang CG, Chen SL. Molecular cloning, subcelluar location and expression profile of signal transducer and activator of transcription 2 (STAT2) from turbot,Scophthalmus maximus. Fish Shellfish Immunol. 2013; 35(4): 1200-1208.

4. Wang N, Yang CG, Sun ZZ, Wang XL, Chen SL. Signal transducer and activator of transcription 3 (STAT3) homologue in turbot (Scophthalmus maximus): Molecular characterization and expression analysis. Fish Shellfish Immunol. 2011; 30(1): 255-262.

5. Wang N, Wang XL, Sha ZX, Tian YS, Chen SL. Establishment, characterization and virus susceptibility of a kidney-derived cell line from southern flounderParalichthys lethostigmaJordan & Gilbert. J Fish Dis. 2011; 34 (1): 81-85.

6. Wang N, Wang XL, Sha ZX, Tian YS, Chen SL. Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus). Fish Physiol Biochem. 2010;36(4):1227-34.

7. Wang XL,Wang N, Sha ZX, Chen SL. Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis). Fish Physiol Biochem. 2010;36(4):1181-9.

8. Wang TT*,Wang N*, Liao XL, Meng L, Liu Y, Chen SL. Cloning, molecular characterization and expression analysis of heat shock cognate 70 (Hsc70) cDNA from turbot (Scophthalmus maximus). Fish Physiol Biochem. 2013; 39(6): 1377-1386.

9. Zheng Y,Wang N, Xie MS, Sha ZX, Chen SL. Establishment and characterization of a new fish cell line from head kidney of half-smooth tongue sole (Cynoglossus semilaevis). Fish Physiol Biochem. 2012; 38(6):1635-43.

10. Sun A, Wang TZ,Wang N, Liu XF, Sha ZX, Chen SL. Establishment and characterization of an ovarian cell line from half-smooth tongue soleCynoglossus semilaevis. J Fish Biol. 2015;86(1):46-59.

11. Tian Y, Jiang J, Song L, Chen Z, Zhai J, Liu J,Wang N, Chen S. Effects of cryopreservation on the survival rate of the seven-band grouper (Epinephelus septemfasciatus) embryos. Cryobiology. 2015 Oct 23. pii: S0011-2240(15)00397-1.

12. Tian Y, Jiang J,Wang N, Qi W, Zhai J, Li B, Liang Y, Chen Y, Yang C, Chen S. Sperm of the giant grouper: cryopreservation, physiological and morphological analysis and application in hybridizations with red-spotted grouper. J Reprod Dev. 2015; 61(4):333-339.

13. Tian YS, Qi WS, Jiang J,Wang N, Wang D, Zhai JM, Chen C, Chen SL. Sperm cryopreservation of sex-reversed seven-band grouper,Epinephelus septemfasciatus. Anim Reprod Sci. 2013; 137: 230-236.

14. Liu WJ, Zhang LY, Shao CW,Wang N, Liu K, Wen HS, Zhang N, Dong ZD, Zhang JJ, Chen SL. Molecular characterization and functional divergence of two Gadd45g homologs in sex determination in half-smooth tongue sole (Cynoglossus semilaevis). Comp Biochem Physiol B Biochem Mol Biol. 2014;177-178:56-64.

15. Yang CG, Liu SS, Sun B, Wang XL,Wang N, Chen SL. Iron-metabolic function and potential antibacterial role of Hepcidin and its correlated genes (Ferroportin 1 and Transferrin Receptor) in turbot (Scophthalmus maximus). Fish Shellfish Immunol. 2013; 34(3): 744-755.

16. Sun A, Chen SL, Gao FT, Li HL, Liu XF,Wang N, Sha ZX. Establishment and characterization of a gonad cell line from half-smooth tongue soleCynoglossus semilaevispseudomale. Fish Physiol Biochem. 2015;41(3):673-83.

17. Zhang B, Wang X, Sha Z, Yang C, Liu S,Wang N, Chen SL. Establishment and characterization of a testicular cell line from the half-smooth tongue sole,Cynoglossus semilaevis. Int J Biol Sci. 2011; 7(4): 452-45

18. Huang J, Chen S, Liu Y, Shao C, Lin F,Wang N, Hu Q. Molecular characterization, sexually dimorphic expression, and functional analysis of 3'-untranslated region of vasa gene in half-smooth tongue sole (Cynoglossus semilaevis). Theriogenology. 2014;82(2):213-24.

19. Meng L, Zhu Y, Zhang N, Liu W, Liu Y, Shao C,Wang N, Chen S. Cloning and characterization of tesk1, a novel spermatogenesis-related gene, in the tongue sole (Cynoglossus semilaevis). PLoS One. 2014 Oct 1;9(10):e107922.

20. Huang JQ, Chen SL, Liu Y, Shao CW, Lin F,Wang N,Hu QM. Molecular characterization, sexually dimorphic expression, and functional analysis of 30-untranslated region of vasa gene in half-smooth tongue sole (Cynoglossus semilaevis). Theriogenology. 2014;82:213-224.

21. Chen S, Zhang G, Shao C, Huang Q, Liu G, Zhang P, Song W, An N, Chalopin D, Volff JN, Hong Y, Li Q, Sha Z, Zhou H, Xie M, Yu Q, Liu Y, Xiang H,Wang N,Wu K, Yang C, Zhou Q, Liao X, Yang L, Hu Q, Zhang J, Meng L, Jin L, Tian Y, Lian J, Yang J, Miao G, Liu S, Liang Z, Yan F, Li Y, Sun B, Zhang H, Zhang J, Zhu Y, Du M, Zhao Y, Schartl M, Tang Q, Wang J. Whole-genome sequence of a flatfish provides insights into ZW sex chromosome evolution and adaptation to a benthic lifestyle. Nat Genet. 2014; 46(3): 253-60.

22. Chen SL, Tian YS, Yang JF, Shao CW, Ji XS, Zhai JM, Liao XL, Zhuang ZM, Su PZ, Xu JY, Sha ZX, Wu PF,Wang N. Artificial gynogenesis and sex determination in half-smooth tongue sole (Cynoglossus semilaevis).Mar Biotechnol (NY). 2009;11(2):243-51.